Bioavailable and Free Testosterone, Blood - Saskatoon
Discipline
Biochemistry
Overview
Description
- Preferred morning collection between 6-10 am
- The panel test includes total testosterone, SHBG, bioavailable testosterone calculated, free testosterone calculated
Alias
|
Specimen Information
Specimen types accepted
- Serum/plasma
Specimen collection container
- Preferred collection container: Gold (gel)
- Alternative collection container: Light green (lithium heparin gel)
Required volume
- Optimal Volume: 4.5 mL Full collection tube
- Minimum Volume: 1.5 mL
(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
Transport and stability
- 7 days at 2-8°C
Rejection criteria
- Specimens received where the collection protocols were not followed
- Pathology and Laboratory Medicine Acceptance and Rejection Criteria
Performance
Methodology
- Total Testosterone measured by Electrochemiluminescence Immunoassay(ECLIA)
- SHBG measured by ECLIA
- The concentrations of free and bioavailable testosterone are derived from mathematical formulae from the Vermeulen et al publication. The Journal of Clinical Endocrinology & Metabolism 1999 Vol. 84, pages 3666 – 3672
Days/times performed
| Availability |
|
| Testing Site |
|
Maximum laboratory time
- In hospital reported within 2 hours
- Community urgent and inter-hospital reported within 4 hours
- Community routine reported within 24 hours
Specimen retention time
- 3 days
Other information
- Bioavailable testosterone includes free and weakly bound (non-SHBG bound) testosterone, and it is the biologically active testosterone.
- Interpret free and bioavailable testosterone results with caution in presence of significant hypoalbuminemia.
Last Updated: October 4, 2024