Bruton Tyrosine Kinase Protein Expression Flow Cytometry, Blood (Referred Out ) - Saskatoon
Discipline
Biochemistry
Overview
Description
- Specimen collection is only available from Monday to Thursday between 0800 and 1200; Specimen needs to be received at St. Paul’s Hospital Laboratory before 14:00 for an overnight courier, otherwise the test will be canceled.
Preferred Specimen Collection
- Recommend pediatric patients to be collected at RUH
Forms Required
- Requisitions must have specific test indicated
- SHR Laboratory Medicine Community Laboratory Requisition Form #101064 or Acute Care Phlebotomy Requisition Form #101062
Alias
- BTK (Bruton’s tyrosine kinase)
- Tyrosine kinase
- X-linked agammablobulinemia (XLA)
Specimen Information
Specimen types accepted
- Whole Blood
Specimen collection container
- Preferred Collection Container-Adult: Yellow (ACD) tube 8.5mL
- Preferred Collection Container-Pediatric: Blue (Sodium Citrate) tube 1.8mL
- Alternate Collection Container: Dark Green (Sodium Heparin) 4.0mL
Collection procedure
- Do not centrifuge, send whole blood to SPH lab; ship at 4oC to the referral site using an overnight courier.
Required volume
- Optimal Volume: Full collection tube
- Minimum Volume: 1 mL whole blood
(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection. Chemistry may be able to do up to 10 tests with 0.75 mL blood.)
- Pediatric Volume: 1.8 mL whole blood
Transport and stability
- Whole blood stable at room temperature for 24 hours
Rejection criteria
Performance
Methodology
- Flow Cytometry
Days/times performed
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Maximum laboratory time
- 2 day turnaround time by referral laboratory
Other information
Additional Comments
- Intracellular staining for BTK protein expression in B cells and monocytes is used to screen males, or female carriers, for X-linked agammaglobulinemia
Last Updated: October 4, 2024