Sulfatase Enzyme Panel, Dried Blood Spot, Blood (Referred Out) - Saskatoon
Discipline
Biochemistry
Overview
Description
Testing ordering requirements
Forms required
- Requisitions must have specific test indicated
- Miscellaneous Specimen Requisition Form #101878 or SHR Laboratory Medicine Community Laboratory Requisition Form #101064 or Acute Care Phlebotomy Requisition Form #101062
Alias
- Multiple Sulfatase Deficiency (Arylsulfatase B(Maroteaux Lamy), N-Acetylgalactosamine-6-sulfatase(Morquio type A), and iduronate 2-sulfatase(Hunter))
Specimen Information
Specimen types accepted
- Whole blood
Specimen collection container
- Preferred collection container: Dark Green (Lithium Heparin) 4.0mL
- Alternative collection container: Dark Green (Sodium Heparin) 4.0mL
- Alternative collection container: Lavender (EDTA) 4.0mL
Required volume
- Optimal volume: 4 mL whole blood
- Minimum volume/Pediatric volume: 1 mL whole blood
Transport and stability
- Whole blood must be received within 24 hours of collection
- Store whole blood at room temperature
- Neonatal Screen Card should be spotted with whole blood within 24 hours of collection
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Once spotted, the Neonatal Screen Card should be allowed to dry for 3-4 hours & shipped to referral laboratory at ambient temperature.
Rejection criteria
- Specimens received where the proper collection protocols were not followed
- Pathology and Laboratory Medicine Acceptance and Rejection Criteria
Testing Information
Relevant clinical history
- Family history
- Must include diagnosis and/or patient symptoms on the requisition
Clinical interpretation
- Result interpretation should be correlated with clinical and other laboratory findings
Performance
Methodology
- Spectrophotometric/ Fluorometric Enzyme Assay
Days/times performed
Availability |
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Testing site |
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Maximum laboratory time
Results reporting
- 2 week turnaround time by referral laboratory
Other information
Additional comments
- The Neonatal Screen card will be spotted when whole blood received in the lab
- This panel includes measurement of 3 sulfatase enzymes including Arylsulfatase B, N-Acetylgalactosamine-6-sulfatase, and iduronate 2-sulfatase
- This test can be used to confirm a suspected Maroteaux Lamy syndrome diagnosis
- This test is considered special testing; request for testing should be supported by a high index of clinical suspicion specific to this disorder
Last Updated: October 23, 2024