Vitamin B6, Plasma (Referred Out) - Saskatoon
Discipline
Biochemistry
Overview
Description
- To assess vitamin B6 nutritional status.
- Patient must avoid vitamin supplements for 24 hours before the sample is drawn. The results from a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status.
- Infants-draw prior to next feeding.
- Non-fasting specimen concentration reflects recent vitamin intake.
Alias
- Pyridoxal Phosphate
Specimen Information
Specimen types accepted
- Plasma
Specimen collection container
- Preferred Collection Container: LAVENDER (4.0 mL K2 EDTA)
Collection procedure
- Separate plasma from cells within 30 min of collection and aliquot into an amber tube.
- Send specimen(s) to St. Paul’s Hospital laboratory frozen or on ice packs.
Required volume
- Optimal Volume: 4.0 mL full collection tube
- Minimum Volume: 1 mL plasma
(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.
Transport and stability
- Vitamin B6 in plasma is stable for 3 days refrigerated and 2 months frozen.
- Ambient: unacceptable
- Send specimen to referral lab frozen
Rejection criteria
- Receipt of specimen not protected from light
- Specimens received where the proper collection protocols were not followed
- Pathology and Laboratory Medicine Acceptance and Rejection Criteria
Testing Information
Clinical interpretation
- The test measures Pyridoxal 5-phosphate, the biologically active form of vitamin B6
Performance
Methodology
- Liquid chromatography/tandem mass spectrometry (LC/MS-MS)
Days/times performed
Availability |
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Testing Site |
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Maximum laboratory time
Results Reporting
- 11 day turnaround time by testing laboratory
Last Updated: October 24, 2024