Disease Association Studies - Provincial
Discipline
Histocompatibility and Immunogenetics (HLA)
Overview
Description
- Preferred collection Monday – Thursday
- Please refer to HLA-B27 in the Lab Medicine Test Catalogue for specific information regarding HLA-B27 testing
Specimen Information
Specimen types accepted
- Whole Blood
Specimen collection container
- Preferred collection container: Lavender top (EDTA) 4 mL
- Alternative collection container: None
Required volume
- Optimal volume: 2- 4 mL lavender top tubes
- Minimum volume: 1 mL
(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
Transport and stability
- Stable for 5 days at 4 - 25˚c
Rejection criteria
- Unlabeled, illegibly-labelled, mislabeled, or inadequately labelled specimens
- Original labeling must be present and visible
- Incomplete, inaccurate, or illegible information on the requisition
- Discrepancies between requisition patient information and specimen label information
- Collected by – required for all collections
- Date and time of collection
- Pathology and Laboratory Medicine Acceptance and Rejection Criteria
Testing Information
Relevant clinical history
- Diagnosis
- Recent transfusions
- Ethnicity
Clinical interpretation
- The presence of the HLA allele(s) associated with a disease alone is not diagnostic of disease. Other clinical factors must be considered to diagnose disease.
Performance
Methodology
- Low and/or high resolution molecular typing (rSSO/SSP)
Days/times performed
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Specimen retention time
- Specimen (Extracted DNA) retained for one year.
Other information
- Please request specific allele required
- Behcet Disease (B51), Birdshot Retinopathy (A29), Celiac (DQ2/DQ8), Narcolepsy (DQB1*06:02)
- Forms required - HLA Test Request Form
Last Updated: March 19, 2024