HLA Antibodies - Provincial
Discipline
Histocompatibility and Immunogenetics (HLA)
Overview
Description
- Inform the HLA laboratory if faster turnaround time is required for investigations of acute graft rejection
- May be requested on bone marrow/stem cell recipients if required for donor selection
Alias
- PRA
- Cytotoxic antibodies
- Donor Specific Antibodies (DSA)
Specimen Information
Specimen types accepted
- Serum
Specimen collection container
- Preferred collection container: Plain red top (Clot Activator) 4 mL
- Alternative collection container: None
Collection procedure
- Collect 2-4 mL plain red top containers
Required volume
- Optimal volume: 8 mL
- Minimum volume: 4 mL
(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)
Transport and stability
- Whole blood: 48 hours at room temperature
- Separated serum: 48 hours at 4°C or 2 years at -20°C or below
Rejection criteria
- Unlabeled, illegibly-labelled, mislabeled, or inadequately labelled specimens
- Original labeling must be present and visible
- Incomplete, inaccurate, or illegible information on the requisition
- Discrepancies between requisition patient information and specimen label information
- Collected by – required for all collections
- Date and time of collection
- Pathology and Laboratory Medicine Acceptance and Rejection Criteria
Testing Information
Relevant clinical history
- Diagnosis
- Transfusion History
- Pregnancies
- Previous Transplants
- Indicate if autoimmune disease is present
- Ethnicity
Performance
Methodology
- Solid phase microarray
Days/times performed
| Availability |
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| Testing Site |
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Maximum laboratory time
- 14 days after routine testing
- 48 hours for investigation of suspected acute antibody mediated rejection
Specimen retention time
- Two years
Other information
Relevant recent medication history
- Immunosuppression therapies
Forms required
Last Updated: March 27, 2024