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HLA-B27 - Provincial

Discipline

Histocompatibility and Immunogenetics (HLA)

Overview

Description

  • May only be requested by Rheumatologists or Ophthalmologists. All others must be cleared.

Alias

  • B27

Specimen Information

Specimen types accepted

  • Whole blood

Specimen collection container

Required volume

  • Optimal volume: 4 mL
  • Minimum volume: 1 mL

(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection.)

Transport and stability

  • Stable for 48 hours at 4-25°C

Rejection criteria

  • Unauthorized ordering physician
  • Unlabeled, illegibly-labelled, mislabeled, or inadequately labelled specimens
  • Original labeling must be present and visible
  • Incomplete, inaccurate, or illegible information on the requisition
  • Discrepancies between requisition patient information and specimen label information
  • Collected by – required for all collections
  • Date and time of collection
  • Pathology and Laboratory Medicine Acceptance and Rejection Criteria

Testing Information

Relevant clinical history

  • Diagnosis
  • History of autoimmune disease

Clinical interpretation

  • The presence of the HLA B27 allele alone is not diagnostic of disease. Associated disease states may be present in the absence of HLA B27. Other clinical factors must be considered to diagnose disease.

Performance

Methodology

  • Low and/or high resolution molecular typing (rSSO/SSP)

Days/times performed

  • Availability - STAT testing not available
  • Testing site - SPH

Specimen retention time

  • Specimen (Extracted DNA) retained for one year.

Other information

  • Clearance required - Physician orders other than from Rheumatologists or Ophthalmologists must be approved by the HIL Laboratory Director (306-655-5205)
  • Forms required - HIL Test Request Form

Last Updated: July 4, 2024