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Kidney Bank Blood - Provincial

Discipline

Histocompatibility and Immunogenetics (HLA)

Overview

Description

  • Drawn quarterly on renal transplant waitlist patients (January, April, July, and October)

Alias

  • KBB
  • HLA antibodies
  • PRA

Specimen Information

Specimen types accepted

  • Serum

Specimen collection container

Required volume

  • Optimal volume: 8 mL
  • Minimum volume: 4 mL

(Submitting the minimum volume makes it impossible to repeat the test or perform confirmatory/reflex testing. In some situations, a minimum volume may require a second collection. 

Transport and stability

  • Unspun serum: 48 hours at room temperature
  • Separated serum: 48 hours at 4°C or 2 years at -20°C or below

Rejection criteria

  • Unlabeled, illegibly-labelled, mislabeled, or inadequately labelled specimens
  • Original labeling must be present and visible
  • Incomplete, inaccurate, or illegible information on the requisition
  • Discrepancies between requisition patient information and specimen label information
  • Collected by – required for all collections
  • Date and time of collection
  • Pathology and Laboratory Medicine Acceptance and Rejection Criteria

Testing Information

Relevant clinical history

  • Diagnosis
  • Transfusion history
  • Pregnancies
  • Previous transplants
  • Indicate if autoimmune disease is present
  • Ethnicity

Performance

Methodology

  • Solid phase microarray

Days/times performed

  • Testing site - SPH

Maximum laboratory time

  • 14 days after routine testing

Specimen retention time

  •  Indefinitely for immediate pre and post transplant sera
  • 2 years for all other sera

Other information

  • Relevant recent medication history - Immunosuppression therapies
  • Additional comments - Serum is retained for future testing requirements 
  • Forms required - HLA Test Request Form

Last Updated: March 27, 2024